POTESMER 2007

Name: POTESMER 2007
Start: 2007-05-01
End: 2007-05-13
Location: Mediterranean Sea, Western Basin

Type	Oceanographic cruise
Ship	Téthys II
Ship owner	CNRS until 2019 - IFREMER since 2020
Dates	01/05/2007 - 13/05/2007
Chief scientist(s)	GREGORI Gérald
	INSTITUT MÉDITERRANÉEN D'OCÉANOLOGIE - UMR 7294 UMR IRD 235 - MARSEILLE 163 avenue de Luminy Bâtiment Méditerranée 13288 Marseille Cedex 9 +33(0)4 86 09 05 00 https://www.mio.osupytheas.fr/fr/linstitut-mediterraneen-doceanologie/presentation-2/
DOI	10.17600/7450080
Objective	This cruise was part of the ANR POTES programme led by Christian TAMBURINI, aiming to characterize the effects of increased hydrostatic pressure on: -processes of organic matter's mineralization and regeneration of biogenic elements in intermediate and deep water; -and structural (biodiversity) and functional (activities) dynamics in prokaryotic communities; - POM fluxes in the water column as well as the speed at which these particles are transformed in the mesopelagic layer. This information will be fed into a model describing the transformation of particles by microorganisms in the entire ocean water column. A number of technological developments were made to meet these objectives. The POTES Mer cruise was mainly designed to test them in situ (new instruments and new analysis protocols). This cruise fell under the ANR POTES programme which has received the marine cluster label awarded by the PACA competitiveness cluster.

Sea/Ocean

Mediterranean Sea, Western Basin

(Zonex 11 et 55 )

Ports

Port of departure : La Seyne-sur-Mer (France)

Port of return : La Seyne-sur-Mer (France)

Limits

North	South	West	East
42.9	42.5	6.1	6.5

Scientific Authority

INSTITUT MÉDITERRANÉEN D'OCÉANOLOGIE - UMR 7294 UMR IRD 235 - MARSEILLE

163 avenue de Luminy

Bâtiment Méditerranée

13288 Marseille Cedex 9

+33(0)4 86 09 05 00

https://www.mio.osupytheas.fr/fr/linstitut-mediterraneen-doceanologie/presentation-2/

Participating bodie(s)

Laboratoire de Microbiologie, Géochimie et Ecologie Marines LMGEM UMR 6117 (Université de la Méditerranée, CNRS, COM-OSU) ; Laboratoire d'Océanographie de Villefranche LOV, Université Pierre et Marie Curie.

Discipline(s)

PHYSICAL OCEANOGRAPHY
BIOLOGICAL OCEANOGRAPHY
CHEMICAL OCEANOGRAPHY
ENVIRONMENT

Code	Label	Quantity	PI
B07	Pelagic bacteria/micro-organisms Production bactérienne, abondances biodiversité	-	TAMBURINI Christian
B16	Benthic bacteria/micro-organisms Biologie moléculaire, activités	4	CUNY Philippe
B64	Gear research Autoéchantillonneur in situ d'oxygène IODA6000	4 deployments	LEFEVRE Dominique
B71	Particulate organic matter (inc POC Etude de la colonisation par les bactéries ; TEP (Avec Stemmann Lars)	2	TAMBURINI Christian
B73	Sediment traps Collecte particules sur godets avec gel (développement technique)	2 samples	STEMMANN Lars
D71	Current profiler (eg ADCP) ADCP de coque	-	GREGORI Gérald
D90	Other physical oceanographic meas. Température, Salinité, O2 dissous, Conductivité, Fluorimétrie	-	GREGORI Gérald
G04	Core soft bottom Carottier multitube	4 cores	CUNY Philippe
H09	Water bottle stations Prélèvements d'eau (bouteilles NISKIN et HPSS) (Avec J.L. Fuda)	24 stations	GREGORI Gérald
H17	Optics (eg underwater light levels) Vidéo-profileur marin PVM	5 stations	STEMMANN Lars
H21	Oxygen Mesures Winkler et auto-échantilloneur in situ IODA 6000	4 stations	LEFEVRE Dominique

Summary of measurements

-The in situ IODA 6000 O2 autosampler was tested at a depth of 2,400 m with continuous measurement over several days of the dissolved O2 concentration (LMGEM).
-Seawater samples (0-2,300 m) were taken in NISKIN bottles with an HPSS (LMGEM) high pressure sampling system to measure bacterial abundance. (Microscopy, CARD-FISH, flow cytometry) & incubations (to measure activity).
-Particle traps (PPS3 type) were deployed at 200 and 400 m (INSU) and new buckets with gel were tested to collect particles.
-Vertical video profiles were made from 0-1,000 m using LOV' PVM marine video profiler to analyze particles in suspension.
-Sediment coring (2,400 m) using the multitube corer (INSU) was done to study biodiversity and sediment quality.

Location map

Bibliography

Publications

Ramondenc Simon, Goutx Madeleine, Lombard Fabien, Santinelli Chiara, Stemmann Lars, Gorsky Gabriel, Guidi Lionel (2016). An initial carbon export assessment in the Mediterranean Sea based on drifting sediment traps and the Underwater Vision Profiler data sets. Deep-sea Research Part I-oceanographic Research Papers, 117, 107-119. https://doi.org/10.1016/j.dsr.2016.08.015

Tamburini Christian, Boutrif Mehdi, Garel Marc, Colwell Rita R., Deming Jody W. (2013). Prokaryotic responses to hydrostatic pressure in the ocean - a review. Environmental Microbiology, 15(5), 1262-1274. https://doi.org/10.1111/1462-2920.12084

Boutrif Mehdi, Garel Marc, Cottrell Matthew T., Tamburini Christian (2011). Assimilation of marine extracellular polymeric substances by deep-sea prokaryotes in the NW Mediterranean Sea. Environmental Microbiology Reports, 3(6), 705-709. https://doi.org/10.1111/j.1758-2229.2011.00285.x

Picheral Marc, Guidi Lionel, Stemmann Lars, Karl David M., Iddaoud Ghizlaine, Gorsky Gabriel (2010). The Underwater Vision Profiler 5: An advanced instrument for high spatial resolution studies of particle size spectra and zooplankton. Limnology And Oceanography-methods, 8, 462-473. Publisher's official version : https://doi.org/10.4319/lom.2010.8.462 , Open Access version : https://archimer.ifremer.fr/doc/00133/24413/

Tapilatu Y., Acquaviva M., Guigue C., Miralles G., Bertrand J.-C., Cuny P. (2010). Isolation of alkane-degrading bacteria from deep-sea Mediterranean sediments. Letters in Applied Microbiology, 50(2), 234-236. Publisher's official version : https://doi.org/10.1111/j.1472-765X.2009.02766.x , Open Access version : https://archimer.ifremer.fr/doc/00303/41417/

Duhamel Solange, Gregori Gerald, Van Wambeke France, Mauriac Romain, Nedoma Jiri (2008). A method for analysing phosphatase activity in aquatic bacteria at the single cell level using flow cytometry. Journal of Microbiological Methods, 75(2), 269-278. https://doi.org/10.1016/j.mimet.2008.06.018

Danovaro Roberto, Dell'Anno Antonio, Corinaldesi Cinzia, Magagnini Mirko, Noble Rachel, Tamburini Christian, Weinbauer Markus (2008). Major viral impact on the functioning of benthic deep-sea ecosystems. Nature, 454(7208), 1084-1088. https://doi.org/10.1038/nature07268

References of Articles published in other Periodicals or Scientific Works acclaimed in the Field

Joux F, Bertrand J-C, De Wit R, Grossi V, Intertagla L, Lebaron P, Michotey V, Normand P, Peyret P, Raimbault P, Tamburini C, Urios L (2011) Chapitre 17: Méthodes d?études des micro-organismes dans l?environnement. In: Presses Université de Pau (ed) Écologie microbienne. Microbiologie des milieux naturels anthropisés. Bertrand JC, Caumette P, Lebaron P, pp 795-872

References of Articles published in periodicals and magazines for the general public

Tamburini C. & POTES Team. POTES - Pressure effects On marine prokaryoTES, an IMBER endorsed project. IMBER Science Highlight, IMBER Update Issue n°10, June 2008.

Tamburini C., Bartlett D.H., Colwell R.R., Deming J.W., Kato C., Patching J.W., Turley C.M. Accurately measuring deep-sea microbial activities and their impacts on biogeochemical cycles. IMBER Science Highlight, IMBER Update Issue n°9, February 2008.

References of International Seminar Communications

Tamburini C. Pressure effects: to be or not to be? Why we must consider it? Oral presentation to the ASLO aquatic science meeting, France, Nice, 25-30 January 2009.

Tamburini C., Boutrif M.*, Garel M., Al Ali B.*, Charrière B., Cuny P., Goutx M., Grégori G., Grossi V., Guigue C., Kirchman D.L., Lee C., Lefèvre D., Pepa S., Panagiotopoulos C., Sempéré R. Effect of pressure on prokaryotic degradation of organic matter according to its quality/composition. Invited speaker to "The Microbial Carbon Pump in the Ocean (MCP)" Working Group (SCOR-WG134), XIAMEN, China, October 27th-30th 2009

Boutrif M., Garel M. Tamburini C. "Pressure effect on degradation of organic matter by deep-sea prokarytic communities". Poster to the First IMBER IMBIZO: ?Integrating biogeochemistry and ecosystems in a changing ocean?, Miami, FL, USA, November 9th-13th 2008.

Sempéré R., Panagiotopoulos C., Tamburini C., Repeta D.J., Charrière B. "Dissolved organic matter (DOM) distribution in the water column of the Ocean: composition and bacterial reactivity." Oral presentation to the First IMBER IMBIZO: "Integrating biogeochemistry and ecosystems in a changing ocean", Miami, FL, USA, November 9th-13th 2008.

Tamburini C., Boutrif M., Charrière B., Cuny P., Garel M., Goutx M., Grégori G., Grossi V., Guigue C., Kirchman D.L., Lee C., Lefèvre D., Repeta D.J., Sempéré R., Wakeham S. "Pressure effects on mineralization of the organic matter by prokaryotes in meso- and bathypelagic waters of the Ocean." Oral presentation to the First IMBER IMBIZO: ?Integrating biogeochemistry and ecosystems in a changing ocean?, Miami, FL, USA, November 9th-13th 2008.

Tamburini C., Goutx M., Guigue C., Garel M., Lefèvre D., Charrière B., Sempéré R., Kirchman D.L., Peterson M.L., Wakeham S. & Lee C. Sinking particles and remineralization rates: controversies and new insights. Solicited speaker to the EurOceans Workshop: "Shedding light on the dark ocean: breaking paradigms in biogeochemistry, diversity and plankton metabolism in the meso- and bathypelagic realm". Noordwijkerhout, Netherlands, February 4th-8th 2008.

DEA or MASTER 2 using campaign data

ROBERT Anne. Minéralisation de la matière organique en milieu profond. LMGEM, 2006.

Thesis using campaign data

AL ALI Badr. Laboratoire LMGEM. Effet de la pression hydrostatique sur la distribution et l'activité (bioluminescence, dégradation de la matière organique) de différents microorganismes marins. Doctorat de l'Université de la Méditerranée, spécialité Océanologie soutenue le 25 janvier 2010. Co-directrice: France Van Wambeke (HDR). Bourse du gouvernement Syrien. Octobre 2006 ? Janvier 2010.

MARTINI Séverine. Laboratoire LMGEM (et M.I.O) La bioluminescence: un proxy biogéochimique en milieu profond? Étude au laboratoire et in situ de la bioluminescence en relation avec les variables environnementales. Octobre 2010 - Décembre 2013. Bourse MNRT.

Tapilatu Yosmina. Laboratoire LMGEM. "Activité hydrocarbonoclaste de procaryotes dans des milieux extrêmes (hyperbares et hypersales)". Avril 2010.

BOUTRIF Mehdi. Laboratoire LMGEM. Dégradation de la matière organique labile et semi-labile dans les domaines méso- et bathypélagique: effet de la pression hydrostatique. Doctorat de l'Université de la Méditerranée, spécialité Océanologie. Co-directeur : Richard Sempéré (HDR). Octobre 2008 - Juillet 2012. Bourse MNRT.

ROBERT Anne. Laboratoire LMGEM. Minéralisation de la matière organique en milieu profond: validation du IODA6000 (In situ Oxygen Deep Auto-sampler). Avril 2007 - Septembre 2012. Contrat EuroSITES. Encadrant secondaire.

DUHAMEL Solange. Laboratoire LMGEM. Rôle des bactéries hétérotrophes et des producteurs primaires dans le cycle biogéochimique du phosphate : couplage des flux de carbone et de phosphate en relation avec la structure fonctionnelle des communautés. Octobre 2004 - Octobre 2007.