IPARO
Type | Oceanographic cruise |
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Ship | Côtes De La Manche |
Ship owner | CNRS until 2019 - IFREMER since 2020 |
Dates | 10/09/2015 - 15/09/2015 |
Chief scientist(s) | COLAS Florent |
RECHERCHES ET DÉVELOPPEMENTS TECHNOLOGIQUES Centre Ifremer Bretagne ZI Pointe du Diable CS 10070 29280 Plouzané +33(0) 2 98 22 41 41 |
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DOI | 10.17600/15012700 |
Objective | Over the past ten years, imaging techniques combined with supervised classification algorithms have proved to be effective in analysing the diversity of plankton populations. The idea is to take images of the organisms contained in a sample, calculate the relevant morphometric parameters on each image and make a classification based on a previously constructed database called a learning set. For two years now, the REM-RDT and ODE-DYNECO units have collaborated in developing two systems: MicroZooCAM and FastCAM. The first is designed to analyze zooplankton analyses and the second phytoplankton. The IPARO cruise's objective is to assess these two pieces of equipment under operational conditions. During the IPARO cruise, a network of stations was performed on the Southern Brittany, Iroise and Bay of Brest zone by taking measurements and samples directly above sectors based on the REPHY network monitoring points (phytoplankton and phycotoxin monitoring network - the same as for the PSEUDOMO1, PSEUDOMO2, PSEUTEC and PHYTEC cruises). The dual objectives of the cruise were to: 1) conduct a technological assessment of the new systems developed at Ifremer; 2) demonstrate the contribution of these methods for the description of phytoplankton structures in Southern Brittany and the Iroise Sea. This large area includes the Loire and Vilaine river plumes, as well as the Iroise zone (Ushant front, Bay of Brest and Bay of Douarnenez). At each station a vertical profile is performed using a DYNECO pelagic profiler. During the profiles made on the study zone, the data and videos acquired in real time by the pelagic profiler made it possible to target the water layers to be sampled for an ex situ analysis of samples by using the FastCAM and MicroZooCAM. A vertical net haul (opening 50 cm in diameter) was also performed in order to obtain a concentrated sample of phytoplankton and thus have an overall view of the species present in the water column. In the case of clearly marked vertical gradients, with a maximum amount of chlorophyll, we also proceeded to take close samples at these gradients with the staged sampler. Part of the samples are collected for taxonomy analyses on shore. |